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Analytical Methods

Monoclonal antibody-based cross-reactive sandwich ELISA for the detection of spp. in milk samples

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Hua Kuang and Chuanlai Xu *

* Corresponding authors

State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, PR China DRESSES Short dresses BABYLON Inexpensive Sale Online Nicekicks Cheap Online 2Q16nh

An immunogen consisting of lipopolysaccharide and bovine serum albumin was prepared by periodate oxidation. Mice sera cross-reacted with strains of the genus . Monoclonal antibodies (mAbs) against the lipopolysaccharide core structure were obtained after cell fusion. Based on mAb 6E8, a cross-reactive sandwich enzyme-linked immunosorbent assay was developed. The detection limit of different strains in the genus ranged from 1.56 × 10 to 1.25 × 10 colony-forming units ml. No cross-reactivity was observed with other bacterial strains tested, including , O6, strain O157 : H7, and . Samples of bovine milk spiked with 1 colony-forming unit ml spp. were analysed following enrichment for 24 h.

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Publication details

The article was received on 23 июл 2015, accepted on 13 сен 2015 and first published on 15 сен 2015

Article type: Paper
DOI: 10.1039/C5AY01923K
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Citation: , 2015, 7 , 9047-9053
Anal. Methods

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John Pendygraft/Getty and its archives were sold to Bryan Goldberg, the founder of Bustle and Bleacher Report, for $1.35 million in a closed-door auction. The initial bidding was led by a $1.13 million offer from marketer Kevin Lee, who intended to turn the site into what he called Pink Tropical Print Halterneck Maxi Dress Pretty Little Thing Discount Shopping Online Outlet Cheap Quality RgIVRt
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