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Analytical Methods

Monoclonal antibody-based cross-reactive sandwich ELISA for the detection of spp. in milk samples

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Hua Kuang and Chuanlai Xu *

* Corresponding authors

State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, PR China xcl@jiangnan.edu.cn +86-510-85329076

An immunogen consisting of lipopolysaccharide and bovine serum albumin was prepared by periodate oxidation. Mice sera cross-reacted with strains of the genus . Monoclonal antibodies (mAbs) against the lipopolysaccharide core structure were obtained after cell fusion. Based on mAb 6E8, a cross-reactive sandwich enzyme-linked immunosorbent assay was developed. The detection limit of different strains in the genus ranged from 1.56 × 10 to 1.25 × 10 colony-forming units ml. No cross-reactivity was observed with other bacterial strains tested, including , O6, strain O157 : H7, and . Samples of bovine milk spiked with 1 colony-forming unit ml spp. were analysed following enrichment for 24 h.

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Publication details

The article was received on 23 июл 2015, accepted on 13 сен 2015 and first published on 15 сен 2015

Article type: Paper
DOI: 10.1039/C5AY01923K
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Citation: , 2015, 7 , 9047-9053
Anal. Methods

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Gawker.com and its archives were sold to Bryan Goldberg, the founder of Bustle and Bleacher Report, for $1.35 million in a closed-door auction. The initial bidding was led by a $1.13 million offer from marketer Kevin Lee, who intended to turn the site into what he called Cheap Sale Really Discount Purchase Midrise trousers Maison Martin Margiela Free Shipping Low Shipping Fee Fashionable Online Sale New Styles acfnRV
a celebrity-driven site that would donate half of its net advertising revenue to charity. Gawker stopped publishing in 2016, after a lawsuit brought against the company by Hulk Hogan and funded by Silicon Valley billionaire Peter Thiel forced it to declare bankruptcy.

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This is not a good sign for the site’s archives. Heading into the auction there appeared to be few—if any—good possible outcomes. But, with the exception of being purchased by Thiel himself (who pulled out of the bidding in April), this is about as ominous as it gets.

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On Wednesday, Federal Judge T.S. Ellis ordered Paul Manafort, former campaign chairman for Donald Trump, to be moved from Northern Neck Regional Jail in Warsaw, Virginia, to an Alexandria, Virginia detention facility. Manafort is in jail pending his trial for a variety of money laundering charges. He was denied bail because of attempted witness tampering .

In ordering the move, the judge wrote the professionals at the Alexandria Detention Center are very familiar with housing high-profile defendants including foreign and domestic terrorists, spies and traitors .”

Manafort’s time at Northern Neck Regional Jail was controversial. The government claimed in filings that Manafort, a key figure in Special Counsel Robert Mueller’s investigation into Russian interference in the 2016 election, was enjoying VIP amenities:

The filings also accuse Manafort of exploiting these privileges by using his laptop to smuggle emails out of jail:

Both the court and the special counsel seem to have lost patience with Manafort’s antics. He’ll be more restricted at his new digs.

Among the unique privileges Manafort enjoys at the jail are a private, self-contained living unit, which is larger than other inmates’ units, his own bathroom and shower facility, his own personal telephone, and his own workspace to prepare for trial. Manafort is also not required to wear a prison uniform. On the monitored prison phone calls, Manafort has mentioned that he is being treated like a “VIP.”

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